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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Dev Biol. 2020 May 20;464(1):35–44. doi: 10.1016/j.ydbio.2020.05.005

Figure 4. The three transcription factors initiate expression in neurons born at different temporal stages.

Figure 4.

All images shown are of 3rd instar larval medulla. (A–A’) UAS-NuLacZ reporter (red) driven by an ey> Gal4 initiates expression at the same time as endogenous Ey expression (blue). (B–B’) UAS-NuLacZ reporter (red) driven by a slp> Gal4 initiates expression at the same time as endogenous Slp expression (blue). (C–C’) UAS-NuLacZ reporter (red) driven by a D> Gal4 initiates expression at the same time as endogenous D expression (blue). (D–F) D>Gal4 is used to drive UAS-NuLacZ (red) in D stage (and all later stage) neuroblasts and progeny. (D) Almost all Ets65A::GFP (green) expressing cells express D>> LacZ. (E) Some Sox102F::GFP (green) expressing cells express D>> LacZ (yellow cells), while others do not (green cells). (F) Some Oc (blue) expressing cells express D>>LacZ (purple cells), while others do not (blue cells). (G-H) slp>Gal4 is used to drive UAS-NuLacZ (red) expression. (G) All neurons expressing Sox102F::GFP (green) also express slp>>LacZ. (H) All neurons expressing Oc (blue) also express slp>>LacZ. (I) ey>Gal4 is used to drive UAS-NuLacZ (red) in Ey stage (and all later stage) neuroblasts and progeny. All Oc (blue) expressing neurons also express LacZ(red).