Fig. 1.

Agronomic traits of osasn1 knockout mutants of rice. (A) Comparison of osasn1-1 and osasn1-2 mutant rice plants and WT controls grown in a paddy field using conventional agricultural practices. The two knockout lines are in different genetic backgrounds, osasn1-1 in Hwayoung cultivar and osasn1-2 in Dongjin cultivar. Plants were transferred to pots from paddy fields, and representative photographs were taken before harvest (scale bar = 20 cm). (B) Grain yield per plant grown in paddy fields (n = 5). (C) Grain thickness (n = 20) of seeds from WT and osasn1 mutants. (D) The 1,000-grain weight from the WT and osasn1 mutants (n = 5). Quantification of nitrogen (E), protein (F) and amino acid (G) concentrations in grains of osasn1 and WT plants. Data represent mean ± SE of five replicates. Significant differences between mutant and WT plants are indicated with an asterisk (*P < 0.05).