TABLE 1.

Thermal stability parameters of B-ZIP domains with arylstibonic acids

NSC13746
Protein	Compound conc., μM	Tma,°C	ΔHm, kcal mol−1	ΔGDib, kcal mol−1	Kaapp(Tm)c
c-Fos|JunD	0	42.5	−45 ± 5	−8.5 ± 0.1
(AP1)	100	66.1	−58 ± 6	−12.6 ± 0.4	6.0 ×106
VBP	0	50.7	−51 ± 6	−9.8 ± 0.2
	100	63.5	−59 ± 7	−12.4 ± 0.3	3.3 ×105
C/EBPα	0	45.8	−56 ± 2	−9.2 ± 0.1
	100	62.7	−65 ± 7	−12.6 ± 0.5	1.7 ×106
C/EBPβ	0	64.4	−71 ± 4	−13.4 ± 0.1
	100	82.4	−77 ± 6	−17.5 ± 0.5	4.9 ×106
CREB	0	49.3	−53 ± 5	−9.7 ± 0.1
	100	77.6	−59 ± 5	−14.5 ± 0.3	6.2 ×108
NSC13782
c-Fos|JunD	0	42.5	−45 ± 5	−8.5 ± 0.1
(AP1)	100	44.7	−47 ± 4	−8.7 ± 0.1	5.9 ×103
VBP	0	50.7	−51 ± 6	−9.8 ± 0.2
	100	56.2	−54 ± 5	−10.8 ± 0.2	2.6 ×104
C/EBPβ	0	64.4	−71 ± 4	−13.4± 0.1
	100	65.2	−69 ± 5	−13.5 ± 0.5	-

^aThe values represent mean of three independent experiments. Error in T_m values was ≤1°C.

^bThe error in the ΔG_Di value is due to error in ΔH_m and ΔCp (1.2 ± 0.2 kcal mol⁻¹) values.

^cBinding constants of compounds for each protein were calculated using equation 3.

Superscript ‘app’ represents the apparent values.