TABLE 1.
Protein | Compound conc., μM | Tma,°C | ΔHm, kcal mol−1 | ΔGDib, kcal mol−1
|
Kaapp(Tm)c |
---|---|---|---|---|---|
c-Fos|JunD | 0 | 42.5 | −45 ± 5 | −8.5 ± 0.1 | |
(AP1) | 100 | 66.1 | −58 ± 6 | −12.6 ± 0.4 | 6.0 ×106 |
VBP | 0 | 50.7 | −51 ± 6 | −9.8 ± 0.2 | |
100 | 63.5 | −59 ± 7 | −12.4 ± 0.3 | 3.3 ×105 | |
C/EBPα | 0 | 45.8 | −56 ± 2 | −9.2 ± 0.1 | |
100 | 62.7 | −65 ± 7 | −12.6 ± 0.5 | 1.7 ×106 | |
C/EBPβ | 0 | 64.4 | −71 ± 4 | −13.4 ± 0.1 | |
100 | 82.4 | −77 ± 6 | −17.5 ± 0.5 | 4.9 ×106 | |
CREB | 0 | 49.3 | −53 ± 5 | −9.7 ± 0.1 | |
100 | 77.6 | −59 ± 5 | −14.5 ± 0.3 | 6.2 ×108 | |
c-Fos|JunD | 0 | 42.5 | −45 ± 5 | −8.5 ± 0.1 | |
(AP1) | 100 | 44.7 | −47 ± 4 | −8.7 ± 0.1 | 5.9 ×103 |
VBP | 0 | 50.7 | −51 ± 6 | −9.8 ± 0.2 | |
100 | 56.2 | −54 ± 5 | −10.8 ± 0.2 | 2.6 ×104 | |
C/EBPβ | 0 | 64.4 | −71 ± 4 | −13.4± 0.1 | |
100 | 65.2 | −69 ± 5 | −13.5 ± 0.5 | - |
The values represent mean of three independent experiments. Error in Tm values was ≤1°C.
The error in the ΔGDi value is due to error in ΔHm and ΔCp (1.2 ± 0.2 kcal mol−1) values.
Binding constants of compounds for each protein were calculated using equation 3.
Superscript ‘app’ represents the apparent values.