Table 3. Primer Sequences Used for qPCR in This Studya.
| genes | forward (5′ to 3′) | reverse (5′ to 3′) |
|---|---|---|
| Ppara | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA |
| Cpt2 | CAGCACAGCATCGTACCCA | TCCCAATGCCGTTCTCAAAAT |
| Pik3ca | TTATTGAACCAGTAGGCAACCG | GCTATGAGGCGAGTTGAGATCC |
| Gsk3b | TGGCAGCAAGGTAACCACAG | CGGTTCTTAAATCGCTTGTCCTG |
| mTOR | ACCGGCACACATTTGAAGAAG | CTCGTTGAGGATCAGCAAGG |
| Cyp2e1 | GGACCTTTCCCAATTCCTTTCTT | TCTTGTGGTTCAGTAGCACCT |
| Cyp7a1 | GGGATTGCTGTGGTAGTGAGC | GGTATGGAATCAACCCGTTGTC |
| Hnf4a | AAGGTGCCAACCTCAATTCATC | CACATTGTCGGCTAAACCTGC |
| Cd36 | ATGGGCTGTGATCGGAACTG | TTTGCCACGTCATCTGGGTTT |
| INS1 | CACTTCCTACCCCTGCTGG | ACCACAAAGATGCTGTTTGACA |
| Pdpk1 | CTGTATGACGCTGTGCCCATT | AAGGGGTTGGTGCTTGGTC |
| Crtc2 | ACAGCCTCAGCGTCCAATC | GTGGAGCCGATGTCCATCAT |
| HSL | CCAGCCTGAGGGCTTACTG | CTCCATTGACTGTGACATCTCG |
| IGF1R | CATGTGCTGGCAGTATAACCC | TCGGGAGGCTTGTTCTCCT |
| IGF1 | CTGGACCAGAGACCCTTTGC | GGACGGGGACTTCTGAGTCTT |
| β-actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
a
Ppara, peroxisome proliferator-activated receptor α. Cpt2, carnitine O-palmitoyltransferase 2. Pik3ca, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α. Gsk3b, glycogen synthase kinase 3 β. mTOR, mammalian target of rapamycin. Cyp2e1, cytochrome P450, family 2, subfamily E, polypeptide 1. Cyp7a1, cholesterol 7alpha-monooxygenase. Hnf4a, hepatocyte nuclear factor 4-α. CD36, cluster of differentiation 36. INS1, insulin 1. Pdpk1, 3-phosphoinositide dependent protein kinase-1. Crtc2, CRE-binding protein (CREB)-regulated transcription coactivator 2. HSL, hormone-sensitive lipase. IGF1, insulin-like growth factor 1. IGF1R, insulin-like growth factor 1 receptor.