Figure 7.

Graph representing an area where the defined criteria are met in white color. The colors are areas where the criteria are not met: red = number of peaks less than 10; blue = number of peaks with resolution ≥1.5 less than 9; orange = number of peaks with tailing ≤1.2 less than 7. Acceptable range of tested conditions is marked with a black rectangle. (a) Graph where x = pump flow rate; y = pH; at final percent of acetonitrile of 95%. (b) Graph where x = pump flow rate; y = final percent of acetonitrile; at pH 7.00. (c) Graph where x = final percent of acetonitrile; y = pH; at a pump flow rate 0.40 mL/min.