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. 2020 Jul 23;15(7):e0235990. doi: 10.1371/journal.pone.0235990

Fig 3. In vivo ROS state in rat liver.

Fig 3

(a) SRA is elevated by 80% and 121% respectively in ethanol-fed rats compared to control groups 1 and 2. Compared to the disease group, I-ol and I-an cause an average decrease by 36% and 43% respectively. (b) Hydrogen peroxide is elevated by 221% and 202% in ethanol-fed rats compared to control groups 1 and 2. Compared to the disease group, I-ol and I-an cause an average decrease by 75% and 78%. UDCA displays a decrease of 32%. (c) GSH is decreased by 63% and 65% in ethanol-fed rats compared to control groups 1 and 2. Compared to the disease group, I-ol and I-an elevate the average amount of GSH by 62% and 94% respectively. (d) TBARS is increased by 76% and 51% in ethanol-fed rats compared to control groups 1 and 2. Compared to the disease group, I-an shows an average decrease of 40% and I-ol at its highest concentration by 50%. (e) GPx activity is decreased by 9% in ethanol-fed rats compared to control group 1. Compared to the disease group, I-ol and I-an elevate the average activity by 17% and 10%, respectively. UDCA shows a 21% decrease in activity. (f) GR activity has decreased by 19% in ethanol-fed rats compared to control groups 2. Compared to the disease group, I-ol and I-an reduce enzymatic activity by 10% and 11% and increase ethanol effect by 41% and 43% respectively. Rats from the disease group (n = 8) as well as from the treatment groups (n = 6 to 8) could not avoid daily alcohol intake because 50 g/l alcohol was mixed in water. Each point represents the individual value of the pathologically relevant parameter of a single rat. The mean value is displayed as horizontal line. Statistical calculation was based on one-way ANOVA and subsequent complex planned contrast or simple contrast analysis. Bonferroni adjustment for multiple comparisons reduced the significance level to * p < 0.0167; ** p < 0.00333; *** p< 0.000333. Dose-dependent effects were specified by Pearson’s correlation coefficient r and linear regression analysis by the coefficient of determination R2 with + p < 0.05; ++ p < 0.01; +++ p < 0.001.