Table 2.

Prevalence of Germline Recombination in Zebrafish Cre Driver Lines that Show Nervous System Recombination

Cre Line Common Name	Full Cre Line Name/Source	Target Gene/Reporter	Breeding Strategya	Germline Recombination Efficiency, Cre from Fatherb	Germline Recombination Efficiency, Cre from Motherb	Reference/Associated Publicationc	Contributorsd
y492-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre-2A-Cerulean)y492	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	A	4.8% (3/63)	0% (0/134)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess
y547-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre)y547	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	B	82.3% (51/62)	38.1% (8/21)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess
y549-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre)y549	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	B	0% (0/58)	6.7% (2/30)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess
y559-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre)y559	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	B	3.4% (3/89)	2.4% (1/42)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess
y546-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre-2A-Cerulean)y546	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	B	51.1% (23/45)	6.2% (4/64)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess
y555-Cre	Et(REX2-SCP1-Ocu.Hbb2:Cre)y555	Tg(actb2:LOXP-EGFP-LOXP-LY-TagRFPT)y272	A	3.2% (3/95)	0% (0/64)	Tabor et al., 2019	Jennifer Sinclair, Harold Burgess

^aBreeding strategy: A: Target^f/f; Cre driver X Target^+/+; B: Target^f/+; Cre driver X Target^+/+.

^bThe numbers (x/y) indicate that x offspring with germline recombination were found from y offspring with the target locus in cumulative data from multiple clutches.

^cThe associated publication reported the generation and characterization of the Cre driver lines but not detailed information about germline recombination.

^dContributors providing information on germline recombination. Please contact the Lead Contact for electronic addresses of principal investigators.