Table 1. Statistics of sequencing data for all libraries.
Library | No. of raw PEs | No. (%) of mergeable PEs | No. (%) of regular sequences | No. (%) of corrected CDR3 clones | % of short DNA fragments |
---|---|---|---|---|---|
C-A | 96496 | 37300 (38.7%) | 58409 (60.5%) | 54915 (94.0%) | 5.2 |
L1-A | 3083953 | 2913875 (94.5%) | 963896 (31.3%) | 879948 (91.3%) | 16.5 |
L2-A | 2554570 | 2426376 (95.0%) | 733248 (28.7%) | 659607 (90.0%) | 18.6 |
L1-AG | 1460605 | 1363539 (93.4%) | 1240721 (84.9%) | 1149563 (92.7%) | 2.7 |
L2-AG | 1378689 | 1273852 (92.4%) | 1174941 (85.2%) | 1081868 (92.1%) | 4.5 |
K1-A | 1949024 | 1778898 (91.3%) | 957574 (49.1%) | 833107 (87.0%) | 9.4 |
K2-A | 3368416 | 3105697 (92.2%) | 1492075 (44.3%) | 1305756 (87.5%) | 13.7 |
K1-AG | 1675636 | 1533437 (91.5%) | 1554608 (92.8%) | 1403029 (90.2%) | 0.5 |
K2-AG | 1547638 | 1409465 (91.1%) | 1423839 (92.0%) | 1264172 (88.8%) | 0.6 |
K1-G | 1526312 | 1434349 (94.0%) | 1208264 (79.2%) | 1077133 (89.1%) | 4.2 |
K2-G | 1509653 | 1427724 (94.6%) | 1135241 (75.2%) | 999473 (88.0%) | 5.5 |
The percentages of mergeable PEs and regular sequences are relative to number of raw PEs. The percentage of CDR3 clones is relative to number of regular sequences. The percentage of short DNA fragments corresponds to the fraction of 250–600 bp fragments in the length analysis.