Table 2.
Summary of the histopathologic, bacteriologic, fluorescence in situ hybridization (FISH), and immunohistochemical results for ovine abortion cases.
| Case† | Histopathology | FISH |
C. fetus isolated‡
(site) |
C. jejuni isolated (site) |
Aerobe bacterial culture (tissues) |
Toxoplasmosis§
(tissues) |
Placenta assessed | Cause of abortion | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Camp. (tissues) |
C. fetus (tissues) |
C. jejuni (tissues) |
C. burnetii placenta |
||||||||
| A1 | Pneumonia, hepatitis | + (li, lu) |
+ (li, lu) |
− (li, lu) |
NA | − (aoc, int) |
NA | NG (li, lu, aoc) | − (br, he, li, lu) |
− | C. fetus |
| A2 | Pneumonia, hepatitis | + (li, lu) |
+ (li, lu) |
− (li, lu) |
NA | − (aoc, int) |
NA | NG (li, lu, aoc) | − (br, he, li, lu) |
− | C. fetus |
| B1 | Hepatitis | − (li, lu) |
− (li, lu) |
− (li, lu) |
NA | NA | NA | NA | − (br, he, li, lu) |
− | Unknown |
| B2 | Placentitis | + (pl) |
+ (pl) |
− (pl, li, lu) |
− | NA | NA | NA | − (br, he, li, lu, pl) |
+ | C. fetus |
| B3 | Placentitis, hepatitis | + (pl) |
+ (pl, li, lu) |
− (pl, li, lu) |
− | NA | NA | NA | − (br, he, li, lu, pl) |
+ | C. fetus |
| C1 | Placentitis, pneumonia | + (pl) |
− (pl, li, lu) |
+ (pl) |
− | − (aoc, pl) |
NA | NG (li, lu, aoc); mixed flora (pl) | − (br, he, li, lu, pl) |
+ | C. jejuni |
| C2 | Hepatitis | − (li, lu) |
− (li, lu) |
− (li, lu) |
NA | − (aoc) |
NA | NG (li, lu, aoc) | − (br, he, li, lu) |
− | Unknown |
| C3 | Placentitis | − (pl) |
− (pl) |
− (pl) |
− | NA | NA | NA | − (pl) |
+ | Unknown |
| C4 | Placentitis | + (pl) |
− (pl) |
+ (pl) |
− | NA | NA | NA | − (pl) |
+ | C. jejuni |
| C5 | Placentitis | + (pl) |
− (pl) |
+ (pl) |
− | NA | NA | NA | − (pl) |
+ | C. jejuni |
| D1 | Placentitis | NA | − (pl, li, lu) |
+ (pl) |
− | − (aoc) |
+¦
(aoc) |
NG (li, lu); mixed flora (pl, aoc) | − (br, he, li, lu, pl) |
+ | C. jejuni |
| D2 | Placentitis | NA | − (pl, li, lu) |
+ (pl, li#) |
− | − (aoc) |
+¦
(aoc) |
NG (li); mixed flora (pl, lu, aoc) | − (br, he, li, lu, pl) |
+ | C. jejuni |
| D3 | Placentitis | NA | − (pl, li, lu) |
+ (pl) |
− | − (aoc) |
+¦
(aoc) |
NG (li); mixed flora (pl, lu aoc) | − (br, pl) |
+ | C. jejuni |
aoc = abomasal contents; br = brain; Camp. = Campylobacter; he = heart; int = intestinal contents; li = liver; lu = lung; NA = data not available; NG = no growth; pl = placenta; − = no/negative; + = yes/positive.
†
Grouped by flock of origin A–D, one abortion case per number.
‡
Culture on thioglycolate agar plates at 37°C under microaerophilic conditions for 48–96 h.
§
Based on histologic and immunohistochemical findings, in cases B2 and B3 combined with real-time PCR.
¦
Isolated in pure culture under anaerobic conditions.
#
Single C. jejuni organisms were detected within the lumen of large hepatic veins.