Table 2. Primer sequences for reverse transcription quantitative polymerase chain reaction.
Gene | Forward (5’ - 3’) | Reverse (5’ - 3’) |
TXNIP | CTGAAGTTACCCGAGTCAAAGC | CTCACCTGTAGCCTGGTCTTCT |
GAPDH | CTGACATGCCGCCTGGAGA | ATGTAGGCCATGAGGTCCAC |
miR-150-5p | CGCCAGGGTTTTCCCAGTCACGACTCCCAACCCTTGTACCAGT | CGCGAGGAGAGAATTAATACGACTCAGTATACGCGCACTGGT |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
Note: TXNIP, thioredoxin-interacting protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; miR, microRNA.