Table 2A. UHPLC gradient elution profiles for each polarity.

HILIC Phase
Time (mins)	% Buffer Composition
	Buffer A - Acetonitrile:Water (95:5) with 5mM Ammonium Formate (pH 3)	Buffer B - Water:Acetonitrile (95:5) with 5mM Ammonium Formate (pH 3)
0	95	5
1	95	5
12	55	45
15	55	45
16	95	5
21	95	5
Reversed Phase (C18)
Time (mins)	% Buffer Composition
	Buffer A - Water with 0.1% Formic Acid	Buffer B - Methanol with 0.1% Formic Acid
0	95	5
2	95	5
9	5	95
12	5	95
13	95	5
16	95	5