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. 2020 Jul 3;12(13):13187–13205. doi: 10.18632/aging.103420

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Copyright © 2020 Mei et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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EA pretreatment inhibited the formation of autophagosomes and autophagolysosomes (yellow arrow) in cortical neurons of rats with CIR injury. (A) Autophagosomes and autophagolysosomes in neurons of the peri-ischemic cortex in the sham group (a and b) and the I/R group (c and d). (B) Autophagosomes and autophagolysosomes in neurons of the peri-ischemic cortex in the I/R + EA group (e and f) and the I/R + EA + EX527 group (g and h). The images of b, d, f, and h indicated the enlarged area of the squares in a, c, e, and g, respectively. Scale bar: a, c, e, g, 5 μm; b, d, f, h, 2 μm.