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. 2020 Jul 1;12(13):12750–12770. doi: 10.18632/aging.103177

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Copyright © 2020 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A short - and long-term evaluation of the clearance effect of ABT263 on OA chondrocytes (P1) in monolayer culture. (A) Schematic of the monolayer experiments in b–f. (B) Gross observation (left; scale bar: 5 mm) and microscope images (right; scale bar: 500 μm) of OA chondrocytes after treatment with 2.5μM ABT263 with SA-β-gal staining. (C) Representative flow cytometric plots measuring cell apoptosis from two independent experiments. (D) Immunofluorescence of HMGB1 in cultured OA chondrocytes at day 3 and day 10. Scale bar: 100 μm. (E) Statistical analysis of the percentage of apoptotic cells and fluorescence intensity of HMGB1 from three independent experiments are shown. (F) Western blot analysis of proteins level for c-caspase3 and p21 at Day 3 and Day 10. (G) qPCR analysis of mRNA levels for CDKN1A and CDKN2A (N = 3 for each group).