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. 2020 Jun 16;12(13):13555–13570. doi: 10.18632/aging.103462

Table 1. Correlation of acylcarnitines with age.

m/z RT (s) SLC Database Common name Median intensity Correlation (ρ) p-value
C18 positive
162.1116 42 2b HMDB00062 Carnitine (C0) 3.60E6 -0.0437 0.5797
454.3869 470 3 LMFA07070010 Eicosenoylcarnitine (C20:1) 2.60E5 0.3249 2.32E-5
452.3712 391 3 LMFA07070011 Eicosadienoylcarnitine (C20:2) 1.78E5 0.2827 2.56E-4
456.4025 465 3 HMDB06460 Arachidyl carnitine (C20:0) 9.27E4 0.282 2.65E-4
312.2154 93 3 HMDB13325 Decadienoylcarnitine (C10:2) 3.01E5 0.2754 3.73E-4
444.3665 348 3 LMFA07070028 Hydroxystearoylcarnitine (C18OH) 7.27E4 0.2439 1.70E-3
310.1998 76 3 LMFA07070016 Decatrienoylcarnitine (C10:3)* 1.03E6 0.2363 2.39E-3
472.3387 335 3 HMDB06510 Docosahexaenoylcarnitine (C22:6) 2.42E4 0.2344 2.60E-3
398.3245 334 3 HMDB06317 Hexadecenoylcarnitine (C16:1)* 6.73E5 0.2341 2.63E-3
218.1375 258 2b HMDB00824 Propionylcarnitine (C3:0) 5.14E4 0.2327 2.79E-3
396.3088 313 3 HMDB13334 Hexadecadienoylcarnitine (C16:2)* 2.30E5 0.2005 0.0103
422.3244 323 3 HMDB06318 Octadecatrienoylcarnitine (C18:3) 2.19E5 0.1947 0.0128
388.3037 286 3 HMDB13166 Hydroxymyristoylcarnitine (C14OH) 1.21E5 0.1735 0.0268
474.3564 347 3 HMDB06321 Docosapentaenoylcarnitine (C22:5) 4.26E4 0.1683 0.0318
420.3089 314 3 HMDB06463 Octadecatetraenoylcarnitine (C18:4) 2.62E4 0.1553 0.0478
464.3352 309 3 LMFA07070036 Hydroxyicosatetraenoylcarnitine (C20:4OH) 4.94E3 0.1539 0.0498
262.1637 258 2b LMFA07070041 Hydroxyvalerylcarnitine (C5OH) 1.51E4 -0.1602 0.0411
246.1687 265 2b HMDB13128 Valerylcarnitine (C5:0)* 5.07E4 -0.1612 0.0398
374.2519 311 3 HMDB13327 Dodecanedioylcarnitine (C12DC) 8.53E4 -0.1914 0.0144
304.1741 257 3 HMDB13328 Pimelylcarnitine (C7DC) 2.97E4 -0.1976 0.0114
302.231 301 3 HMDB13288 Nonanoylcarnitine (C9:0) 6.07E4 -0.2174 5.30E-3
Anion exchange
310.2014 57 3 LMFA07070016 Decatrienoylcarnitine (C10:3)* 3.30E5 0.2421 1.85E-3
398.3265 76 3 HMDB13207 Hexadecenoylcarnitine (C16:1)* 3.68E5 0.1945 0.0128
426.3576 111 3 HMDB06351 Octadecenoylcarnitine (C18:1) 2.55E6 0.1808 0.0209
396.3108 65 3 HMDB13334 Hexadecadienoylcarnitine (C16:2)* 1.21E5 0.1723 0.0279
369.2828 59 3 HMDB13331 Tetradecadienoylcarnitine (C14:2) 1.20E5 0.1682 0.0318
288.2169 63 2b HMDB00791 Octanoylcarnitine (C8:0) 5.17E5 0.1632 0.0374
372.3109 71 3 HMDB05066 Myristoylcarnitine (C14:0) 2.51E5 0.1547 0.0486
286.2013 570 2b HMDB13324 Octenoylcarnitine (C8:1) 2.72E4 -0.1681 0.032
246.1698 581 2b HMDB13128 Valerylcarnitine (C5:0)* 7.13E3 -0.1778 0.0232
330.2272 584 3 HMDB13202 Ketodecanoylcarnitine (Oxo-C10) 2.08E3 -0.1905 0.0149

Correlation analyzed by Spearman (ρ) and significance of correlation (p-value) are shown for each acylcarnitine detected on both C18 and anion exchange columns. Mass-to-charge ratio (m/z), retention time (RT), Schymanski level of confidence (SLC), and Human Metabolome Database (HMDB) or Lipid Maps (LMFA) database identifier are given for each acylcarnitine. Although no significant correlation was found for free carnitine (C0), values are given for reference. A SLC of 3 indicates a putative identification using LCMS data, whereas a SLC of 2b indicates a probable match using additional diagnostic MS/MS data. All acylcarnitines were detected as an M + H adduct. *, acylcarnitine identified by both C18 and anion exchange.