Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus; male and female) | C57BL/6J | National Institute of Aging Charles River colony | RRID:IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | mCAT | Rabinovitch Lab; PMID:15879174 |
RRID:IMSR_JAX:016197; Tg(CAG-OTC/CAT)4033Prab |
Now available at The Jackson Lab |
| Transfected construct (M. musculus) | AAV9-mCAT | Duan Lab, PMID:19690612 |
AV.RSV.MCAT | Adeno-associated virus construct to transfect and express mCAT transgene |
| Antibody | Anti-OXPHOS (Rabbit polyclonal) | Abcam | ab110413 RRID:AB_2629281 |
(1:500) |
| Antibody | Anti-Troponin I (Rabbit polyclonal) | Cell Signaling Technology | #4002 RRID:AB_2206278 |
(1:1000) |
| Antibody | Anti-pSer23/24-Troponin I (Rabbit polyclonal) | Cell Signaling Technology | #4004 RRID:AB_2206275 |
(1:1000) |
| Antibody | Anti-pSer150-Troponin I (Rabbit polyclonal) | ThermoFisher | PA5-35410 RRID:AB_2552720 |
(1:1000) |
| Antibody | Anti- cMyBP-C (Mouse monoclonal) | Santa Cruz SC-137237 | SC-137237 RRID:AB_2148327 |
(1:1000) |
| Antibody | Anti-pSer282-cMyBP-C (Rabbit polyclonal) | Enzo | ALX-215–057 R050 RRID:AB_2050502 |
(1:2000) |
| Antibody | anti-p19 (Rabbit polyclonal) | LSBio | LS-C49180 RRID:AB_1192824 |
(1:300) |
| Antibody | anti-p16 (Rabbit polyclonal) | Abcam | ab211542 | (1:300) |
| Antibody | Donkey anti-Rabbit Secondary Antibody, HRP | ThermoFisher | A16035 RRID:AB_2534709 |
(1:10000) |
| Antibody | Goat anti-Mouse Secondary Antibody, HRP | ThermoFisher | A16072 RRID:AB_2534745 |
(1:10000) |
| Peptide, recombinant protein | SS-31 peptide (Elamipretide) | Stealth BioTherapeutics | 3 µg/g body weight/day | |
| Commercial assay or kit | OxiSelect protein carbonyl ELISA kit | Cell Biolabs | STA-310 | |
| Commercial assay or kit | ImmPRESS-VR Anti-Rabbit IgG HRP Polymer Detection Kit | Vector Laboratories | MP-6401–15 | |
| Commercial assay or kit | Seahorse XF Cell Mito Stress Test Kit | Aligent/Seahorse Bioscience | 103015–100 | |
| Commercial assay or kit | MitoSOX Red | ThermoFisher | M36008 | |
| Commercial assay or kit | MitoPY1 | Fisher Scientific/Tocris Bioscience | 44-281-0 | |
| Commercial assay or kit | MitoTracker Green | ThermoFisher | M7514 | |
| Commercial assay or kit | MitoTracker Deep Red | ThermoFisher | M22426 | |
| Commercial assay or kit | JC-1 Dye | ThermoFisher | T3168 | |
| Commercial assay or kit | BCA protein assay | Thermo Scientific | 23225 | |
| Commercial assay or kit | Pierce Reversible Protein Stain Kit for PVDF Membranes | Thermo Scientific | 24585 | |
| Commercial assay or kit | SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Scientific | 34580 | |
| Software, algorithm | Graphpad Prism | Graphpad | RRID:SCR_002798 | |
| Software, algorithm | AlphaView Software | ProteinSimple | ||
| Software, algorithm | Metaboanalyst 4.0 |
www.metaboanalyst.ca; PMID:29762782 |
RRID:SCR_015539 | |
| Software, algorithm | Topograph | MacCoss Lab software; PMID:22865922 | ||
| Software, algorithm | ComplexHeatmap |
https://github.com/jokergoo/ComplexHeatmap
PMID:27207943 |
RRID:SCR_017270 |