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. 2020 Jun 9;53(7):e12823. doi: 10.1111/cpr.12823

FIGURE 4.

FIGURE 4

Homeobox C6 (HOXC6) is testified to be a downstream target of miR‐361‐3p in cervical cancer (CC). A, Through utilizing starBase, 12 mRNAs that predicted to have binding capacity with miR‐361‐3p in PITA, miRmap, microT and PicTar databases were screened out. B, The expression level of HOXC6 in CC tissues and neighbouring non‐tumour tissues was obtained from GEPIA. C, RT‐qPCR was used to detect the expression of HOXC6 in CC tissues and neighbouring non‐tumour tissues. D, The expression of HOXC6 in CC cells and H8 cells was detected via RT‐qPCR and Western blot analyses. E, Pearson's correlation analysis revealed the correlation between HOXC6 and miR‐361‐3p (or BBOX1 antisense RNA 1 [BBOX1‐AS1]). F, A binding site between miR‐361‐3p and HOXC6 was predicted via starBase. G, The efficiency of HOXC6 overexpression in SiHa and HeLa cells was examined by RT‐qPCR. H and I, The interaction between RNAs (BBOX1‐AS1, miR‐361‐3p and HOXC6) was confirmed by luciferase reporter and RIP assays. J, The expression of HOXC6 in different groups was analysed via RT‐qPCR. * P < .05, ** P < .01