FIGURE 5.

BBOX1 antisense RNA 1 (BBOX1‐AS1) promotes the mRNA stability of homeobox C6 (HOXC6) via HuR in cervical cancer (CC). A, The mRNA expression of HOXC6 was detected by utilizing RT‐qPCR after SiHa and HeLa cells were treated with Actinomycin D. B, Through searching starBase, 4 RBPs (U2AF2, ELAVL1, LARP4B and RBM10) were found to be able to bind with BBOX1‐AS1 (or HOXC6). C, RNA pull‐down assay demonstrated that only ELAVL1 (also known as HuR) bound with BBOX1‐AS1 in SiHa and HeLa cells. D, RIP assay verified that HuR could bind with BBOX1‐AS1 (or HOXC6) in SiHa and HeLa cells. E, Efficiency of indicated shRNAs for HuR silencing was identified through RT‐qPCR in SiHa and HeLa cells. F, Stability of HOXC6 mRNA was assessed in transfected cells treated with Actinomycin D was analysed in several different time points. G, RIP assays were utilized to analyse the changes in the interaction between HOXC6 mRNA and HuR under BBOX1‐AS1 silence. H, Effect of BBOX1‐AS1 inhibition on HuR expression was evaluated by RT‐qPCR and Western blot analyses. I, The efficiency of HOXC6 knockdown was measured by RT‐qPCR. J, The expression of HOXC6 in SiHa and HeLa cells transfected with different plasmids was detected via RT‐qPCR. ^** P < .01