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. 2020 Jun 17;53(7):e12809. doi: 10.1111/cpr.12809

Table 1.

Primers and amplification conditions used for qRT‐PCR

Genes Accession (NCBI) Primer sequences Size(bp) Temperature (°C)
Cat NM_009804.2

F: CAGCGACCAGATGAAGCAGT

R: CCTCAAAGTATCCAAAAGCACC

236 59
Gpx1 NM_001329528.1

F: GGAGAATGGCAAGAATGAAGA

R: AGGAAGGTAAAGAGCGGGTG

135 58
Prdx2 NM_011563.6

F: GATGGTGCCTTCAAGGAAATCA

R: CCGTGGGGCAAACAAAAGTG

97 60
Prdx6 NM_001303408.1

F: CGCCAGAGTTTGCCAAGAG

R: TCCGTGGGTGTTTCACCATTG

115 60
Sod1 NM_011434.1

F: GGGTTCCACGTCCATCAGTA

R: TTGCCCAGGTCTCCAACAT

128 59
Sod2 NM_013671.3

F: CAGACCTGCCTTACGACTATGG

R: CTCGGTGGCGTTGAGATTGTT

113 61
Bax NM_007527.3

F: ATGCGTCCACCAAGAAGCTGAG

R: CCCCAGTTGAAGTTGCCATCAG

166 62
Bak NM_007523.3

F: CAACCCCGAGATGGACAACTT

R: CGTAGCGCCGGTTAATATCAT

101 59
Caspase3 NM_001284409.1

F: GACTGGGATGAACCACGACCC

R: TCTGACTGGAAAGCCGAAAC

205 60
Caspase9 NM_015733.5

F: CTGGGAAGGTGGAGTAGGAC

R: GCGGTGGTGAGCAG

189 56
β‐actin NM_007393.5

F: TCGTGGGCCGCCCTAGGCAC

R: TGGCCTTAGGGTTCAGGGGGG

243 59
Gapdh NM_001289726.1

F: GACAAAATGGTGAAGGTCGGT

R: GAGGTCAATGAAGGGGTCG

120 58