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. 2020 Jan 24;26(8):4205–4220. doi: 10.1038/s41380-020-0649-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a (Left panels) Representative immunoblots and quantitative analysis of SERT levels in the mPFC of male (upper) and female (lower) mice. Male mice: AdipoR1^f/f, n = 6; AdipoR1^f/f;ePet-Cre, n = 6. Female mice: AdipoR1^f/f, n = 7. AdipoR1^f/f;ePet-Cre, n = 7. (Right panels) Representative brain sections immunostained for 5-HT and quantitative analysis of the intensity of 5-HT-positive fibers in the mPFC of male (upper) and female (lower) mice. Male: 14 sections from three AdipoR1^f/f mice; 14 sections from three AdipoR1^f/f;ePet-Cre mice. Female: 14 sections from three AdipoR1^f/f mice; 13 sections from three AdipoR1^f/f;ePet-Cre mice. Scale bar = 50 µm. b (Left panels) Representative immunoblots and quantitative analysis of SERT levels in the hippocampus of male (upper) and female (lower) mice. Male mice: AdipoR1^f/f, n = 6; AdipoR1^f/f;ePet-Cre, n = 6. Female mice: AdipoR1^f/f, n = 7. AdipoR1^f/f;ePet-Cre, n = 7. (Right panels) Representative brain sections immunostained for 5-HT and quantitative analysis of the intensity of 5-HT-positive fibers in the hippocampus of both male (upper) and female (lower) mice. Male: 24 sections from three AdipoR1^f/f mice; 23 sections from three AdipoR1^f/f;ePet-Cre mice. Female: 21 sections from three AdipoR1^f/f mice; 24 sections from three AdipoR1^f/f;ePet-Cre mice. Scale bar = 100 µm for low magnification and 50 µm for high magnification. c Forced swim test performed 30 min after fluoxetine (5 or 10 mg kg⁻¹, i.p.) or desipramine (10 mg kg⁻¹, i.p.) injection in male (left) and female (right) mice. Male mice: AdipoR1^f/f + saline, n = 7; AdipoR1^f/f + fluoxetine (5 mg kg⁻¹), n = 9; AdipoR1^f/f + fluoxetine (10 mg kg⁻¹), n = 7; AdipoR1^f/f + desipramine, n = 9; AdipoR1^f/f;ePet-Cre + saline, n = 7; AdipoR1^f/f;ePet-Cre + fluoxetine (5 mg kg⁻¹), n = 9; AdipoR1^f/f;ePet-Cre + fluoxetine (10 mg kg⁻¹), n = 8; AdipoR1^f/f;ePet-Cre + desipramine, n = 7. Female mice: AdipoR1^f/f + saline, n = 8; AdipoR1^f/f + fluoxetine, n = 7; AdipoR1^f/f + desipramine, n = 10; AdipoR1^f/f;ePet-Cre + saline, n = 7; AdipoR1^f/f;ePet-Cre + fluoxetine, n = 7; AdipoR1^f/f;ePet-Cre + desipramine, n = 10. *P < 0.05, **P < 0.01, ***P < 0.001 compared with AdipoR1^f/f littermate controls or saline-treated groups. d Schematic diagram illustrating the alterations in 5-HT system components and antidepressant responses induced by the loss of AdipoR1 in 5-HT neurons.