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. 2020 Jun 17;39(30):5292–5306. doi: 10.1038/s41388-020-1360-y

Fig. 1. Effect of miR-1300 expression on proliferation and cell death in U251 and KNS42 cell lines.

Fig. 1

a Cell cycle time course analyzed by flow cytometry using propidium iodide (PI) loading as a measure of DNA content. b Binucleation phenotype scoring following following 72 h of treatment and staining with DAPI and Phalloidin Alexafluor488 (Actin) and the corresponding representative image. All experiments were performed in triplicate. The results are normalized to a scrambled-mimic control. c Cell death measured by flow cytometry where Annexin negative (An−)/PI negative (PI−) = live cells, An+ = early-mid apoptotic cells, An+ PI+ = mid-late apoptotic cells and An− PI + = necrotic cells. Statistical significance is expressed as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.