Skip to main content
. 2020 Jun 17;39(30):5292–5306. doi: 10.1038/s41388-020-1360-y

Fig. 2. Effect of miR-1300 expression on proliferation and cell death in GSC cultures GBM1 and GBM4.

Fig. 2

a Cell cycle time course analyzed by flow cytometry using propidium iodide (PI) loading as a measure of DNA content. b Cell death measured by flow cytometry where Annexin negative (An−), PI negative (PI−) = live cells, An+ PI− = early-mid apoptotic cells, An+ PI+ = mid-late apoptotic cells and An− PI+ = necrotic cells. c, d Binucleation phenotype scoring following staining with DAPI and Phalloidin Alexafluor488 (Actin) (3–6 images per condition, representing at least 100 cells) was performed on images taken on the Operetta imaging platform (×10 objective). All experiments were performed in triplicate. The results are normalized to a scrambled-mimic control. Statistical significance is expressed as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.