Fig. 5. ECT2 is a direct target of miR-1300.

Direct targeting assessed by 3′UTR luciferase assay in the KNS42 cells (a) and GBM4 GSCs (b). 3′ECT2 represents the wild-type 3′UTR sequence, 3′ECT2-mt represents the 3′UTR sequence containing two point mutations in the predicted binding site for the miR-1300 seed sequence. Ectopic expression of ECT2 rescues miR-1300 induced cytokinesis failure. Binucleation phenotype scoring (3–6 FOV per condition, representing at least 100 cells) was performed on images taken on the Operetta imaging platform (×10 objective) in KNS42 (c) and GBM1 (d). All experiments were performed in triplicate. The results are normalized to the double control: “MIM control + Control vector” representing the scrambled-mimic combined with the empty vector devoid of the ECT2 expression cassette. Statistical significance is expressed as follows: **p < 0.01 and ****p < 0.0001.