Fig. 6. Exposure of synchronized CMK cells to SU6656 concomitantly induces an increase in the levels of miR-1300 and a decrease of its target, ECT2.

a Cells were synchronized using Monastrol 25 mM for 24 h prior to exposure to SU6656 5 mM. b Increase in multinuclear megakaryocytic cells (MKC) as a result of endomitosis undergone by megakaryoblastic cells (MKB) was measured by immunofluorescence in response to SU6656. Cells were stained with DAPI and TOTO-3. Nine fields of view were analyzed using an algorithm designed in the Columbus software to discriminate objects based on their size (b). Statistical significance is expressed as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.