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. 2020 Jun 17;39(30):5292–5306. doi: 10.1038/s41388-020-1360-y

Fig. 7. Ectopic expression of miR-1300 specifically affects GBM stem-like cells but not their differentiated counterparts.

Fig. 7

a Effect on the cell cycle was analyzed by flow cytometry 120 h post transfection using Propidium Iodide (PI) loading as a measure of DNA content. b Cell death measured also 120 h post transfection by flow cytometry where Annexin negative (An−), PI negative (PI−) = live cells, An+ PI− = early apoptotic cells, An+ PI+ = mid/late apoptotic cells and An− PI + = necrotic cells. c Binucleation phenotype scoring (3–6 images per condition, representing at least 100 cells) was performed on images taken on the Operetta imaging platform (×10). All experiments were performed in triplicate. The results are normalized to a scrambled-mimic control without BMP4 exposure. Statistical significance is expressed as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.