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. 2020 Jul 23;10:12353. doi: 10.1038/s41598-020-69317-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Linear discriminant analysis (LDA) effect size plots showing the differences in uterine microbiota among cows diagnosed in the fifth week postpartum as healthy (n = 8), subclinical endometritis (SCE; n = 8; < 50% purulent vaginal discharge and > 5% endometrial neutrophils (PMN)), or with clinical endometritis (CE; n = 5; ˃ 50% purulent vaginal discharge and > 5% endometrial PMN). Histograms show the LDA effect size computed for features at the phyla (A1, A2) and genera (B1, B2) levels. Enriched features for healthy and SCE are indicated with positive LDA scores (green), and enriched features in CE cows are indicated with negative LDA scores (red). Only features with P ˃ 0.05 and an effect size cut-off of 3.5 are plotted.