Fig. 2.

Chronic Mn-induced chronic neuroinflammation and loss of dopaminergic neurons. Adult male SD rats were given injections of saline, Mn²⁺ 1 and 5 mg/kg, i.p. every 10 days for 150 days. The brains were collected and SNpc region was double stained for dopaminergic neurons (THir-strain, red) and microglia (Iba1-stain, green). Representative photos were shown. b Relative cell counts in chronic Mn-induced chronic neuroinflammation and loss of dopaminergic neurons. Adult male SD rats were given injections of saline, Mn²⁺ 1 and 5 mg/kg, i.p. every 10 days for 150 days. The activation of microglia (Iba1+ cells) and loss of dopaminergic neurons (THir cells) were quantified. Data are mean + SEM of 3 rats. *Significantly different from Controls, p < 0.05