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. 2020 Jul 23;11(7):562. doi: 10.1038/s41419-020-02785-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR analysis of circ-0046263 expression after treatment with three siRNAs. b CCK-8 assay was performed to assess NPC cell growth vitality. c The proliferative capacity in 5–8F and CNE-2 cells were determined via colony-formation assay. d, e Wound-healing assay and Transwell assay were performed to assess migration and invasion ability of cells transfected with si-circ-0046263 or si-NC. Scale bars, 100 μm. f Expression of EMT-related proteins was detected by Western blot analysis. Results are shown as mean ± SD, and the data were represented by three independent experiments. *P < 0.05; **P < 0.01.