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. 2020 Jul 23;11(7):562. doi: 10.1038/s41419-020-02785-3

Fig. 3. Circ-0046263 functions as a miRNA sponge in NPC cells.

Fig. 3

a, b mRNA and protein expressions of the host gene were detected by qRT-PCR and Western blot after knocking down circ-00466263. c, d Nuclear separation and FISH shown subcellular localization of circ-0046263. Scale bars, 50 μm. e PITA and miRnada predicted miRNAs targeting circ-0046263. f Luciferase reporter assay detected luciferase activity after co-transfecting with four miRNA mimics and circ-0046263-WT or circ-0046263-MUT reporter plasmid. g miR-133a-5p was identified in the circ-0046263-RISC complex. Control and miR-133a-5p-inhibitor cell lysates were used for RNA-IP with anti-AGO2 antibody. Circ-0046263 and miR-133a-5p expression levels were detected by qRT-PCR. h, i Silencing of circ-0046263 did not affect the expression of miR-133a-5p, and miR-133a-5p did not affect the expression of circ-0046263. All data are presented as mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01.