. 2020 Jul 23;10:12263. doi: 10.1038/s41598-020-67844-3

Table 2.

Endpoint dose response for cAMP generation and arrestin recruitment stimulated by vasopressin and oxytocin via the V₂ vasopressin receptor.

Ligand	cAMP			Arrestin
Ligand	E_max NFU	E_max % vaso	− log L₅₀ (L₅₀, nM)	E_max NFU	E_max % vaso	− log L₅₀ (L₅₀, nM)
Vasopressin	0.51 ± 0.09^a	100 ± 0	10.05 ± 0.08^b (0.089)	0.16 ± 0.02^c	100 ± 0	7.68 ± 0.04^d (21)
Oxytocin	0.56 ± 0.12^a	110 ± 5	9.44 ± 0.13^b (0.36)	0.16 ± 0.03^c	97 ± 4	6.75 ± 0.08^d (180)

The responses at a single time point (18 min after ligand application, 22 min after experiment initiation) were measured using genetically-encoded biosensors^26,82 as described in Methods. See Fig. 6 for raw data. The data were fit to a sigmoid curve equation⁸³ to determine the maximal initial rate (IR_max, the upper plateau, equivalent to k_τ) and L₅₀ (concentration of ligand giving an initial rate 50% of IR_max). Data values are mean ± SEM, n = 3. Curve fit results for each experiment are provided in the “Curve fit results” Supplementary file. NFU is normalized fluorescence units. Ligand parameters were compared statistically by paired two-tailed t-test, with the following p values: ^a0.258, ^b0.0496, ^c0.390, ^d0.0101.