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. 2020 Jul 23;10:12255. doi: 10.1038/s41598-020-69166-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human serum IgE recognition assays. (A) nDer p 1, rproDer p 1 and proDerp1αS comparative detection by serum IgE of Der p 1-positive allergic patients (#1–15) and non-allergic (C1, C2) individuals by mean of indirect ELISA. Black arrows indicate selected sera for in vitro humRBL-2H3 degranulation and viability assays. Asterisks indicate selected patients for BAT and basophils cytotoxicity assays. (B) Inhibition of serum binding to nDer p 1 by rproDer p 1 (open circles), the derived immunotoxin chimera (grey circles) and using the allergen nDer p 1(black circles) as a control of maximum inhibition. Graph representation shows the resulting media ± standard deviation for three sera (#1, 3, 10). All experiments were done by duplicate.