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. 2020 Jul 23;10:12255. doi: 10.1038/s41598-020-69166-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Basophil degranulation. (A) Basophil activation test dose dependant profiles for nDer p 1, rproDer p 1, proDerp1αS and α-sarcin performed with basophils from Der p 1-allergic patients (n = 5). Individual profiles of each patient are presented in Supplementary Figure S3. (B) nDer p 1, rproDer p 1 and proDerp1αS individual and medium EC₅₀ representation for the five Der p 1 allergic patient. Black horizontal gross bars represent the EC₅₀ means while upward deviation bars the sample standard deviation.