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. 2020 Jul 23;11(7):566. doi: 10.1038/s41419-020-02795-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The mRNAs and lncRNAs binding to PML-RARα (left panel). qRT-PCR validated MALAT1 binding to PML-RARA (right panel). b Schematic diagram showing the procedure of MS2-Trap assays. c PML-RARα interacted with MALAT1 in HEK-293T cells. The three sections of MALAT1 were named as MALAT1-1, MALAT1-2, MALAT1-3 respectively. d The mRNAs binding to PML-RARα. qRT-PCR confirmed the binding (right panel). e The mRNA FISH assay for detecting the interaction of PML-RARα and its own mRNA in HEK-293T cells in which PML-RARα-EGFP over-expressed. PML-RARα-EGFP (green) represents the fusion protein and PML-CY3 (red) represents mRNA transcripts. Scale bar represents 4 μm. f Reanalysis of the GES13159 data set with patient samples classified into many subtypes, including AML with t(11q23)/MLL, AML1-ETO, PML-RAR, and CBFb-MYH11. MALAT1 expression presented the highest levels in the AML patient subgroups with fusion proteins. g, h RIP assays followed by qRT-PCR analysis for the interaction of MALAT1 and the fusion protein mRNAs with fusion proteins (MLL-ENL, AML1-ETO, and MLL-AF9) in HEK-293T cells. Data are shown as the means ± s.e.m.; n = 3 independent experiments.