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. 2020 Jul 23;11(7):566. doi: 10.1038/s41419-020-02795-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Screening of mRNA export-related proteins that are specifically responsible for PML-RARα mRNA. b PML-RARα was diminished after knockdown of SRSF3 or YTHDC1 in NB4 cells. The data were obtained from three independent experiments. c Wright–Giemsa staining assays showed that phenotypes of the nuclei changed to a lobulated morphology after inhibiting YTHDC1 or SRSF33. Scale bar represents 20 μm. d Representative graphs for the flow cytometric analysis of myeloid markers of CD11b, CD14, and CD15 in NB4 when the YTHDC1 and SRSF3 were knocked down. Histogram plots show the statistical values. Error bars reflect ± SEM in three independent experiments. Error bars reflect ± SEM in three independent experiments (***p < 0.001). e YTHDC1 and SRSF3 transcripts expressions do not change significantly after knockdown of MALAT1 in NB4 cells. f Fusion mRNA transport process was dependent on YTHDC1. RNA FISH assay for testing PML-RARα mRNA transport by transfecting constructs of PML-RARα-EGFP-12×MS2bs and YTHDC1-MS2 upon knocking down MALAT1 in HEK-293T cells. Scale bar represents 4 μm. Data are shown as the means ± s.e.m.; n = 3 independent experiments.