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. 2020 Jul 23;10:12358. doi: 10.1038/s41598-020-69096-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LCM of brain microvessels yields transcriptomic datasets enriched in endothelial and pericyte markers. (A,B) Representative lectin-stained mouse brain sections before (A) and after (B) LCM. Scale bars: 50 μm. (C,D) Transcript abundance [log₂(TPM + 1)] of endothelial, pericyte, and neural genes in mouse (C) and human (D) whole brain and LCM microvessel samples. Lines connect datapoints from matched LCM microvessel and whole brain samples. *P < 0.05 versus whole brain, DESeq2 Wald test with Benjamini–Hochberg correction. Exact P-values are provided in Supplementary Table S4.