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. 2020 Jul 16;8:388. doi: 10.3389/fped.2020.00388

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Copyright © 2020 Merino, Slisarenko, Taylor, Falkenstein, Gilbert, Bohm, Blanchard, Ardeshir, Didier, Kim and Kuroda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of selected PBMC immunophenotyping parameters. Age binning was completed for samples from animals with average ages of 1 week (0–2 weeks old), 4 weeks (3–5 weeks old), 12 weeks (11–13 weeks old), 24 weeks (22–26 weeks old), 36 weeks (34–38 weeks old), and 48 weeks (46–50 weeks old) of the Colony MR cohort (blue), Colony NR (green), and Research NR cohort (orange). Results are shown for; (A,E) CD20+ B cells, (B,F) CD1c+ dendritic cells, (C,G) CM CD4+ T cells, and (D,H) CM CD8+ T cells. Panels (A–D) were plotted using box and whiskers depicting median, minimum and maximum values and panels (E–H) applied statistical analyses of results (mean ± st. dev.) from the Colony MR animals using Kruskal-Wallis test coupled with Dunn's multiple comparisons to illustrate how CBC parameters vary with age. *P < 0.05; **P < 0.01; ***P < 0.001.