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. 2020 Jul 16;11:1335. doi: 10.3389/fimmu.2020.01335

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Copyright © 2020 Barbu, Dominical, Mendelsohn and Thein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium ionophore treatment induces a rapid change in histone citrullination in both normal and decondensed neutrophil nuclei in the absence of extracellular calcium. Neutrophils purified from three healthy donors were left in DPBS (blue), or treated for the indicated times with vehicle only, DMSO (red), or A23187 4 μM (green) (A) Percent neutrophils with decondensed nuclei. (B) Percent neutrophils with normal and decondensed nuclei positive for H4cit3. (C) Similarity scores for DNA (Hoechst stain) and MPO, marking co-localization of two distinct cellular compartments, nucleus, and type I granules. Paired t-test was used to compare untreated with vehicle and ionophore 4 μM, respectively at each time point; Anova for panel ionophore-mediated response in (C) *P < 0.05, **P < 0.01.