Figure 6.

The level of nuclear super-condensation caused by the NETs inducers varies with the stimulus. Purified healthy neutrophils were treated with the NETs stimuli for the shown lengths of time. Percent cells with super-condensed 1-lobe nuclei were identified and quantified by plotting nucleus/whole cell Area vs. Morphology Area (nuclear stain—Hoechst). An example of these analysis features is shown in Figure S8A. A variable number of experiments were conducted for each stimulus. Filled symbols, RPMI control; empty symbols, stimulus. Paired t-test was used to compare the response in untreated and treated neutrophils. *P < 0.05, **P < 0.01.