Table 1.

NETs stimuli.

Reagents	Purified neutrophils (2 × 106 in 500 μl)
	RPMI only (control)	Hemin 20 μM	Hemin 5 μM	LPS 1 μg/ml	PMA 100 nM	PMA 20 nM	IL-8 50 pg/ml
(μl)	100	100	100	100	100	100	100
Reaction vol. (μl)	600	600	600	600	600	600	600
PFA 8% (μl)	600	600	600	600	600	600	600