Figure 2.

Experimental procedure. (A) CD115⁺ bone marrow (BM)-derived monocytes from wildtype mice were isolated and enriched by magnetic-activated cell sorting (MACS) anti-CD115 microbead selection column. (B) Isolated monocytes were then split into one of four treatment groups, defined by the sequence with which cytokines were added to fresh culture medium on Days 0 and 3. The treatment conditions were as follows: (1) IL-34 alone followed by IL-34 alone (IL-34), (2) M-CSF alone followed by M-CSF alone (M-CSF), (3) M-CSF alone followed by IL-34 alone (M-CSF → IL-34), and (4) mix of M-CSF and IL-34 followed by the same combination (M-CSF + IL-34). (C) On Day 6, cultures were either left untreated or stimulated with fibrillar or oligomeric (f/o)Aβ₄₂ for 30 min, after which time cells in both cultures were fixed and stained for markers of interest. (D) Legend for the treatment groups.