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Primer and probe sequences of TREC, KREC, and β-actin.
| Name | Sequence (5′->3′) | ||
|---|---|---|---|
| TREC | Forward | TREC-Forward | CACATCCCTTTCAACCATGCT |
| Reverse | TREC-Reverse | GCCAGCTGCAGGGTTTAGG | |
| Probe | TREC-FAM | ACACCTCTGGTTTTTGTAAAGGTGCCCACT | |
| KREC | Forward | KREC-Forward | TCCCTTAGTGGCATTATTTGTATCACT |
| Reverse | KREC-Reverse | AGGAGCCAGCTCTTACCCTAGAGT | |
| Probe | KREC-HEX | TCTGCACGGGCAGCAGGTTGG | |
| β-actin | Forward | ACTB-Forward | ATTTCCCTCTCAGGCATGGA |
| Reverse | ACTB-Reverse | CGTCACACTTCATGATGGAGTTG | |
| Probe | ACTB-Cy5 | GTGGCATCCACGAAACTA |
