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. 2020 Jul 23;10:12372. doi: 10.1038/s41598-020-69256-9

Figure 4.

Figure 4

PLZ treatment restores the loss of axonal varicosities under a HFD. Reconstructed 3D volume of axons with varicosities and collateral branches in the myenteric plexus in STD-Veh (a,d,e), HFD-Veh (b,f,g), and HFD-PLZ (c,h,i) mice. Varicosity areas (d,f,h, arrows) and collateral branches (b, white arrows) and axonal mitochondria (e,g,i, arrowheads) are shown. (j) HFD-Veh mice had fewer axonal varicosities than STD-Veh mice, whereas HFD-PLZ mice contained more axonal varicosities with numerous synaptic vesicles than HFD-Veh mice. (k) The varicosity minor axis was increased in HFD-PLZ mice compared to that in STD-Veh and HFD-Veh mice. (l) Mitochondrial minor axis and surface area (m) were not significantly different. (n) Mitochondrial volume was increased in HFD-Veh mice when compared to that in STD-Veh mice. (o) Lysosome number decreased in HFD-PLZ mice compared to that in HFD-Veh mice. n (STD = 3, HFD = 3, HFD-PLZ = 3) = 30 axons each (j,o–q), 158, 17, 79 varicosities (k), or 30 mitochondria (l–n). Graphs show the mean ± SD (j,l–n) for normally distributed data or the medians (k,o–q, bars) with quartile range (k,o–q, whiskers) for other data. Statistical significances were calculated by ANOVA followed by Bonferroni post-hoc tests (j,l–n) or Kruskal–Wallis tests (k,o). *P < 0.05, **P < 0.01, ****P < 0.0001. Bars: 1 µm.