FIGURE 3.

Biofilm production in P. phymatum wild-type and mutant strains. The cells were incubated in nitrogen limited (A)BM minimal medium at 30°C for 5 days before biofilm production (OD₅₇₀) was quantified. Three independent biological replicates of each strain were assayed. Wild-type (WT) and the complemented (bceN-COMP) strains produced significantly more biofilm than the mutant (bceN-IM) and the mutant containing the empty vector (bceN-pBBR), respectively. Error bars indicate the standard error of the mean (SEM). ****p-value < 0.0001.