Fig. 4.

Immunoblots of structural changes to human plasma FN treated with MPO/H₂O₂/SCN⁻. Human plasma FN was separated using SDS-PAGE under non-reducing (A, C) or reducing (B, D) conditions, and then transferred onto PVDF membranes and probed with either the mouse monoclonal anti-FN CBF antibody (A, B; A17; 1:10000) or anti-FN HBF antibody (C, D; A32; 1:2000), followed by HRP conjugated anti-mouse HRP secondary antibody (1:2000). Blots were developed with chemiluminescence reagent and data labelled as follows: black arrow = dimer/higher aggregates, and white arrow = monomer band.