Fig. 5.

Calcein-AM fluorescence and proliferation of HCAEC incubated on human plasma FN treated with MPO/H₂O₂/Cl⁻ or MPO/H₂O₂/SCN⁻. Surface-bound human plasma FN (0.02 μM) was left untreated (0x H₂O₂), treated with MPO/Cl⁻ (A, D), MPO/20 μM SCN⁻ (B, E) or MPO/500 μM SCN⁻ (C, F), and increasing concentration of H₂O₂ (0, 8, 32 μM) and incubated for 2 h at 37 °C before incubation with HCAEC pre-stained with calcein-AM and subsequent detection of fluorescence to quantify cell adhesion (A, B, C). Plates were then washed prior to addition of growth media and HCAEC were left to recover over 48 h. MTS reagent was added into each well and absorbance was measured at 490 nm after 3 h for cell proliferation assay (D, E, F). All data are presented as a percentage of the control, with no added H₂O₂. Error bars are ±SD from three technical replicates obtained from each of three independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparison post hoc tests to determine significance. Statistical significance is identified as follow: * = p < 0.05.