Table 1.

Expression primers sequences and PCR cycle conditions.

Gene (ID)	Primer	5′- 3′ Sequence	Reaction Condition
Gene expression
RUNX2 (12393)	Forward	GGACGAGGCAAGAGTTTCA	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	TGGTGCAGAGTTCAGGGAG
BSP (103993)	Forward	GTACCGGCCACGCTACTTTCT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	GTTGACCGCCAGCTCGTTTT
ALP (11647)	Forward	GAAGTCCGTGGGCATCGT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	CAGTGCGGTTCCAGACATAG
MMP9 (17395)	Forward	TGTGCCCTGGAACTCACACGAC	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	ACGTCGTCCACCTGGTTCACCT
MMP2 (17390)	Forward	AACTTTGAGAAGGATGGCAAGT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	TGCCACCCATGGTAAACAA
FAK (14083)	Forward	TCCACCAAAGAAACCACCTC	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	ACGGCTTGACACCCTCATT
SRC (17977)	Forward	TCGTGAGGGAGAGTGAGAC	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	GCGGGAGGTGATGTAGAAAC
TIMP2 (21858)	Forward	GCAACAGGCGTTTTGCAATG	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	CGGAATCCACCTCCTTCTCG
INTEGRIN-Β1 (16412)	Forward	CTGATTGGCTGGAGGAATGT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	TGAGCAATTGAAGGATAATCATAG
COFILIN (12631)	Forward	CAGACAAGGACTGCCGCTAT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	TTGCTCTTGAGGGGTGCATT
PP2A (19052)	Forward	ATGGGCCTCTCTCCCATTCT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	CATGCACAGGGAGTGACAGT
BGLAP2 (12097)	Forward	AGACTCCGGCGCTACCTT	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	CTCGTCACAAGCAGGGTTAAG
SPP1 (20750)	Forward	TTTGCTTTTGCCTGTTTGGC	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	CAGTCACTTTCACCGGGAGG
DMP1 (13406)	Forward	CCCAAAACAGGAAAGGATCA	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	ACCAGACTGCTTCTCCTCCA
GAPDH (14433)	Forward	AGGCCGGTGCTGAGTATGTC	95 °C - 15 s; 60 °C - 30 s; 72 °C - 30 s
	Reverse	TGCCTGCTTC ACCACCTTCT