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. 2020 Jul 16;11:804. doi: 10.3389/fphys.2020.00804

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Copyright © 2020 Zhang, Song, Hong, Shen, Sui and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-9 targets and negatively-regulates SDC2. (A) Predicted upstream miRNAs of SDC2 by the DIANA, TarBase, TargetScan, and microRNA.org databases; (B) Putative miR-9 binding sites in the 3’UTR of SDC2 mRNA following bioinformatics analysis; (C) mmu-miR-9 binding with the 3’UTR of SDC2 mRNA confirmed by dual-luciferase reporter gene assay; and (D) hsa-miR-9 binding with the 3’UTR of SDC2 mRNA confirmed by dual-luciferase reporter gene assay. *p < 0.05 vs. cells treated with mimic NC; n = 8 for mice upon each treatment. All data were measurement data and expressed as mean ± standard deviation. Comparisons were analyzed using the unpaired t-test. The experiment was repeated 3 times.