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Viral injection selectively labeled ChAT-positive neurons in the PPT. (A) EYFP-expressing PPT neurons (green arrows) following AAV injection. (B) Same region as shown in (A), but imaged to show immunostaining for ChAT. (C) Merged image showing colocalization of ChAT antibody and viral EYFP expression (green arrows). Scale = 100 μm.
