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. 2020 Jul 23;10:12305. doi: 10.1038/s41598-020-69268-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extraction and detection of Pf NAs from used RDTs. (A) Recovery rates of Pf DNA from RDTs collected in asymptomatic Tanzanian school children. (B) Comparison of extraction performance of four protocols based on Cq values of the human rnasep gene. (C) Association of parasite densities and Cq values of freshly prepared Pf strains (PfDD2, PfHB3 and PfNF54). Gray colour indicates failed detection. (D) Correlation between parasite densities of serially diluted PfIS and Cq values for PlasQ targets. Red coloured dots represent samples where amplification failed.