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. 2020 Jul 23;10:12305. doi: 10.1038/s41598-020-69268-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adaptation of ENAR protocol for analyzing large numbers of barcoded RDTs. (A) Malaria indicator survey conducted including a detailed questionnaire. (B) Malaria prevalence is determined by RDT followed by storage of barcode-labelled RDTs. (C) Sorting and tracking of RDTs using smartphone apps. (D–F) High throughput protocol for extraction of NAs from RDTs using the ENAR approach (Image courtesy of LJNovaScotia/Pixabay). (G) Detection and quantification of Pf and non-Pf malaria parasite. (H) Automated analysis of qPCR data using ELIMU-MDx. (I) Genotyping of pfk13 propeller region for drug resistance monitoring.