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. 2020 Jul 23;11(7):559. doi: 10.1038/s41419-020-02766-6

Fig. 5. MiR-628-5p suppresses the progression of gastric cancer.

Fig. 5

a CCK-8 to detect the proliferation of MGC-803 that transfected miR-628-p mimic or negative control. b The cell cycle of cells in a are detected by flow cytometry. c, d The colony formation of cells in a and the clone number was counted. e Detect the proliferation of AGS that transfected miR-628-p inhibitor or negative control. f The cell cycle of cells in e are detected by flow cytometry. g, h The colony formation of cells in e and the clone number was counted. i, j Transwell test to detect the migration and invasion of treated HGC-27. The migrated and invaded cells were counted. k, l Transwell test to detect the migration and invasion of treated AGS. The migrated and invaded cells were counted. m The protein expression of PIN1 downstreams after intervened miR-628-5p in MGC-803 and AGS. The data are shown as the mean ± s.d. (n = 3) in cell lines. **p < 0.01, ***p < 0.001. Scale bars: 200 μm.